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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2023-02-28, 10:46 CET based on data in: /zpool/fastq/fastqc


        General Statistics

        Showing 441/441 rows and 3/6 columns.
        Sample Name% Dups% GCM Seqs
        SRR7819727_1
        59.5%
        49%
        37.3
        SRR7819727_2
        57.8%
        49%
        37.3
        SRR7819728_1
        53.9%
        49%
        33.4
        SRR7819728_2
        51.5%
        50%
        33.4
        SRR7819729_1
        57.6%
        51%
        32.0
        SRR7819729_2
        55.4%
        51%
        32.0
        SRR7819730_1
        59.4%
        51%
        34.3
        SRR7819730_2
        58.4%
        51%
        34.3
        SRR7819731_1
        63.0%
        52%
        34.1
        SRR7819731_2
        60.6%
        52%
        34.1
        SRR7819732_1
        62.5%
        50%
        36.1
        SRR7819732_2
        59.9%
        51%
        36.1
        SRR7819733_1
        65.8%
        50%
        35.4
        SRR7819733_2
        65.9%
        51%
        35.4
        SRR7819734_1
        65.3%
        51%
        33.8
        SRR7819734_2
        62.6%
        51%
        33.8
        SRR7819735_1
        61.0%
        50%
        39.5
        SRR7819735_2
        59.7%
        50%
        39.5
        SRR7819736_1
        59.9%
        50%
        35.6
        SRR7819736_2
        58.7%
        50%
        35.6
        SRR7819737_1
        64.7%
        51%
        33.8
        SRR7819737_2
        64.0%
        52%
        33.8
        SRR7819738_1
        62.0%
        50%
        34.5
        SRR7819738_2
        58.4%
        51%
        34.5
        SRR7819739_1
        62.3%
        49%
        35.1
        SRR7819739_2
        60.4%
        50%
        35.1
        SRR7819740_1
        59.0%
        49%
        37.9
        SRR7819740_2
        55.2%
        50%
        37.9
        SRR7819741_1
        62.6%
        49%
        34.4
        SRR7819741_2
        60.8%
        50%
        34.4
        SRR7819742_1
        62.2%
        50%
        32.1
        SRR7819742_2
        61.1%
        50%
        32.1
        SRR7819743_1
        60.0%
        50%
        32.9
        SRR7819743_2
        59.3%
        51%
        32.9
        SRR7819744_1
        61.5%
        50%
        34.8
        SRR7819744_2
        60.3%
        51%
        34.8
        SRR7819745_1
        58.6%
        50%
        41.3
        SRR7819745_2
        57.5%
        51%
        41.3
        SRR7819746_1
        65.6%
        51%
        35.1
        SRR7819746_2
        62.0%
        52%
        35.1
        SRR7819747_1
        56.9%
        50%
        35.4
        SRR7819747_2
        55.6%
        50%
        35.4
        SRR7819748_1
        61.7%
        51%
        34.1
        SRR7819748_2
        59.4%
        52%
        34.1
        SRR7819749_1
        61.2%
        51%
        34.3
        SRR7819749_2
        62.0%
        51%
        34.3
        SRR7819750_1
        61.5%
        49%
        35.0
        SRR7819750_2
        60.3%
        49%
        35.0
        SRR7819751_1
        63.9%
        51%
        31.6
        SRR7819751_2
        61.5%
        52%
        31.6
        SRR7819752_1
        61.8%
        50%
        31.9
        SRR7819752_2
        61.6%
        50%
        31.9
        SRR7819753_1
        59.4%
        49%
        36.0
        SRR7819753_2
        58.1%
        50%
        36.0
        SRR7819754_1
        58.7%
        48%
        42.1
        SRR7819754_2
        53.5%
        49%
        42.1
        SRR7819755_1
        63.6%
        49%
        43.0
        SRR7819755_2
        62.3%
        50%
        43.0
        SRR7819756_1
        57.0%
        50%
        35.7
        SRR7819756_2
        55.2%
        50%
        35.7
        SRR7819757_1
        54.7%
        49%
        37.9
        SRR7819757_2
        52.3%
        49%
        37.9
        SRR7819758_1
        60.4%
        50%
        33.4
        SRR7819758_2
        59.2%
        50%
        33.4
        SRR7819759_1
        65.7%
        50%
        31.5
        SRR7819759_2
        62.0%
        51%
        31.5
        SRR7819760_1
        56.8%
        49%
        34.8
        SRR7819760_2
        53.9%
        49%
        34.8
        SRR7819761_1
        80.2%
        52%
        34.6
        SRR7819761_2
        78.6%
        53%
        34.6
        SRR7819762_1
        70.6%
        51%
        35.6
        SRR7819762_2
        70.5%
        51%
        35.6
        SRR7819763_1
        70.4%
        49%
        36.2
        SRR7819763_2
        69.5%
        50%
        36.2
        SRR7819764_1
        64.3%
        51%
        35.6
        SRR7819764_2
        60.7%
        52%
        35.6
        SRR7819765_1
        54.6%
        49%
        35.4
        SRR7819765_2
        52.4%
        49%
        35.4
        SRR7819766_1
        58.6%
        49%
        33.5
        SRR7819766_2
        56.0%
        49%
        33.5
        SRR7819767_1
        63.1%
        50%
        36.3
        SRR7819767_2
        62.1%
        50%
        36.3
        SRR7819768_1
        61.5%
        49%
        36.2
        SRR7819768_2
        58.8%
        49%
        36.2
        SRR7819769_1
        58.2%
        48%
        38.9
        SRR7819769_2
        55.1%
        49%
        38.9
        SRR7819770_1
        63.0%
        50%
        36.1
        SRR7819770_2
        58.9%
        51%
        36.1
        SRR7819771_1
        62.8%
        50%
        36.0
        SRR7819771_2
        61.4%
        51%
        36.0
        SRR7819772_1
        58.1%
        49%
        38.0
        SRR7819772_2
        55.5%
        49%
        38.0
        SRR7819773_1
        56.4%
        49%
        38.0
        SRR7819773_2
        53.8%
        49%
        38.0
        SRR7819774_1
        65.4%
        50%
        32.3
        SRR7819774_2
        67.3%
        51%
        32.3
        SRR7819775_1
        57.0%
        49%
        35.5
        SRR7819775_2
        58.0%
        49%
        35.5
        SRR7819776_1
        56.3%
        50%
        32.5
        SRR7819776_2
        58.2%
        50%
        32.5
        SRR7819777_1
        62.5%
        50%
        35.0
        SRR7819777_2
        61.5%
        50%
        35.0
        SRR7819778_1
        58.2%
        49%
        36.2
        SRR7819778_2
        60.1%
        49%
        36.2
        SRR7819779_1
        58.8%
        51%
        35.8
        SRR7819779_2
        59.2%
        52%
        35.8
        SRR7819780_1
        62.0%
        48%
        33.3
        SRR7819780_2
        62.5%
        48%
        33.3
        SRR7819781_1
        55.9%
        49%
        40.5
        SRR7819781_2
        57.7%
        50%
        40.5
        SRR7819782_1
        80.2%
        52%
        33.9
        SRR7819782_2
        80.0%
        54%
        33.9
        SRR7819783_1
        57.1%
        49%
        33.4
        SRR7819783_2
        54.2%
        49%
        33.4
        SRR7819784_1
        62.7%
        49%
        38.0
        SRR7819784_2
        60.6%
        50%
        38.0
        SRR7819785_1
        61.4%
        50%
        36.3
        SRR7819785_2
        59.0%
        50%
        36.3
        SRR7819786_1
        59.3%
        51%
        36.8
        SRR7819786_2
        60.4%
        52%
        36.8
        SRR7819787_1
        61.1%
        49%
        35.3
        SRR7819787_2
        58.8%
        49%
        35.3
        SRR7819788_1
        56.0%
        51%
        33.2
        SRR7819788_2
        56.2%
        51%
        33.2
        SRR7819789_1
        60.1%
        50%
        36.3
        SRR7819789_2
        57.8%
        51%
        36.3
        SRR7819790_1
        59.5%
        49%
        34.2
        SRR7819790_2
        56.9%
        50%
        34.2
        SRR7819791_1
        59.7%
        50%
        37.2
        SRR7819791_2
        57.4%
        51%
        37.2
        SRR7819792_1
        58.9%
        50%
        35.1
        SRR7819792_2
        55.2%
        50%
        35.1
        SRR7819793_1
        56.8%
        50%
        31.5
        SRR7819793_2
        54.1%
        51%
        31.5
        SRR7819794_1
        57.7%
        49%
        34.2
        SRR7819794_2
        54.1%
        50%
        34.2
        SRR7819795_1
        54.7%
        50%
        31.5
        SRR7819795_2
        54.1%
        51%
        31.5
        SRR7819796_1
        65.6%
        52%
        33.9
        SRR7819796_2
        65.5%
        53%
        33.9
        SRR7819797_1
        60.7%
        50%
        36.7
        SRR7819797_2
        58.2%
        50%
        36.7
        SRR7819798_1
        61.0%
        50%
        33.4
        SRR7819798_2
        61.1%
        50%
        33.4
        SRR7819799_1
        57.9%
        49%
        33.0
        SRR7819799_2
        55.2%
        50%
        33.0
        SRR7819800_1
        65.4%
        52%
        35.8
        SRR7819800_2
        64.3%
        53%
        35.8
        SRR7819801_1
        59.9%
        49%
        35.6
        SRR7819801_2
        56.8%
        50%
        35.6
        SRR7819802_1
        60.6%
        50%
        34.5
        SRR7819802_2
        61.1%
        51%
        34.5
        SRR7819803_1
        57.1%
        49%
        34.5
        SRR7819803_2
        53.9%
        49%
        34.5
        SRR7819804_1
        58.3%
        49%
        35.0
        SRR7819804_2
        55.4%
        50%
        35.0
        SRR7819805_1
        60.7%
        50%
        33.6
        SRR7819805_2
        60.7%
        51%
        33.6
        SRR7819806_1
        60.2%
        49%
        31.6
        SRR7819806_2
        57.0%
        50%
        31.6
        SRR7819807_1
        58.3%
        50%
        31.5
        SRR7819807_2
        55.0%
        50%
        31.5
        SRR7819808_1
        64.2%
        49%
        39.3
        SRR7819808_2
        60.9%
        50%
        39.3
        SRR7819809_1
        64.8%
        51%
        35.2
        SRR7819809_2
        62.7%
        52%
        35.2
        SRR7819810_1
        60.0%
        49%
        34.8
        SRR7819810_2
        57.4%
        50%
        34.8
        SRR7819811_1
        67.7%
        53%
        38.7
        SRR7819811_2
        68.1%
        53%
        38.7
        SRR7819812_1
        57.6%
        50%
        31.6
        SRR7819812_2
        54.8%
        50%
        31.6
        SRR7819813_1
        58.0%
        49%
        32.2
        SRR7819813_2
        53.8%
        49%
        32.2
        SRR7819814_1
        68.7%
        52%
        40.1
        SRR7819814_2
        67.3%
        52%
        40.1
        SRR7819815_1
        58.3%
        50%
        32.1
        SRR7819815_2
        55.2%
        51%
        32.1
        SRR7819816_1
        59.2%
        49%
        39.9
        SRR7819816_2
        56.3%
        50%
        39.9
        SRR7819817_1
        65.3%
        50%
        34.0
        SRR7819817_2
        62.4%
        51%
        34.0
        SRR7819818_1
        57.4%
        49%
        35.2
        SRR7819818_2
        53.8%
        50%
        35.2
        SRR7819819_1
        61.6%
        51%
        35.2
        SRR7819819_2
        55.1%
        51%
        35.2
        SRR7819820_1
        60.7%
        50%
        36.8
        SRR7819820_2
        58.5%
        50%
        36.8
        SRR7819821_1
        60.7%
        51%
        34.8
        SRR7819821_2
        57.7%
        51%
        34.8
        SRR7819822_1
        61.3%
        50%
        39.9
        SRR7819822_2
        59.3%
        50%
        39.9
        SRR7819823_1
        56.8%
        50%
        35.1
        SRR7819823_2
        53.1%
        50%
        35.1
        SRR7819824_1
        70.3%
        51%
        44.3
        SRR7819824_2
        69.5%
        51%
        44.3
        SRR7819825_1
        72.7%
        50%
        29.7
        SRR7819825_2
        68.4%
        50%
        29.7
        SRR7819826_1
        68.9%
        50%
        29.9
        SRR7819826_2
        67.2%
        50%
        29.9
        SRR7819827_1
        72.7%
        50%
        32.9
        SRR7819827_2
        71.1%
        50%
        32.9
        SRR7819828_1
        88.9%
        51%
        60.9
        SRR7819828_2
        89.2%
        52%
        60.9
        SRR7819829_1
        75.5%
        51%
        26.7
        SRR7819829_2
        75.8%
        52%
        26.7
        SRR7819830_1
        75.8%
        49%
        48.3
        SRR7819830_2
        74.3%
        49%
        48.3
        SRR7819831_1
        70.5%
        51%
        36.4
        SRR7819831_2
        70.8%
        51%
        36.4
        SRR7819832_1
        75.4%
        50%
        33.0
        SRR7819832_2
        73.3%
        50%
        33.0
        SRR7819833_1
        67.9%
        50%
        44.3
        SRR7819833_2
        67.5%
        51%
        44.3
        SRR7819834_1
        61.9%
        51%
        32.7
        SRR7819834_2
        60.2%
        51%
        32.7
        SRR7819835_1
        78.3%
        52%
        39.4
        SRR7819835_2
        74.9%
        52%
        39.4
        SRR7819836_1
        71.4%
        49%
        32.0
        SRR7819836_2
        67.4%
        49%
        32.0
        SRR7819837_1
        74.1%
        51%
        39.9
        SRR7819837_2
        72.9%
        51%
        39.9
        SRR7819838_1
        68.7%
        53%
        30.6
        SRR7819838_2
        67.5%
        53%
        30.6
        SRR7819839_1
        67.3%
        50%
        30.2
        SRR7819839_2
        63.0%
        51%
        30.2
        SRR7819840_1
        76.2%
        49%
        37.0
        SRR7819840_2
        73.6%
        49%
        37.0
        SRR7819841_1
        71.8%
        51%
        34.5
        SRR7819841_2
        68.6%
        52%
        34.5
        SRR7819842_1
        70.1%
        50%
        33.6
        SRR7819842_2
        67.5%
        51%
        33.6
        SRR7819843_1
        71.9%
        49%
        39.2
        SRR7819843_2
        68.8%
        49%
        39.2
        SRR7819844_1
        69.8%
        49%
        39.1
        SRR7819844_2
        67.4%
        49%
        39.1
        SRR7819845_1
        71.2%
        51%
        30.9
        SRR7819845_2
        67.8%
        51%
        30.9
        SRR7819846_1
        71.6%
        49%
        34.1
        SRR7819846_2
        69.2%
        50%
        34.1
        SRR7819847_1
        65.0%
        51%
        40.6
        SRR7819847_2
        63.0%
        51%
        40.6
        SRR7819848_1
        78.9%
        48%
        28.4
        SRR7819848_2
        76.4%
        48%
        28.4
        SRR7819849_1
        80.3%
        50%
        41.2
        SRR7819849_2
        79.2%
        50%
        41.2
        SRR7819850_2
        61.6%
        49%
        13.6
        SRR7819852_1
        74.0%
        50%
        39.0
        SRR7819852_2
        70.0%
        51%
        39.0
        SRR7819853_1
        74.4%
        49%
        43.6
        SRR7819853_2
        73.8%
        50%
        43.6
        SRR7819854_1
        67.1%
        51%
        32.9
        SRR7819854_2
        64.7%
        51%
        32.9
        SRR7819855_1
        73.3%
        50%
        39.5
        SRR7819855_2
        72.0%
        51%
        39.5
        SRR7819856_1
        73.0%
        50%
        37.9
        SRR7819856_2
        71.1%
        51%
        37.9
        SRR7819857_1
        72.5%
        51%
        37.1
        SRR7819857_2
        70.5%
        52%
        37.1
        SRR7819858_1
        67.0%
        50%
        34.6
        SRR7819858_2
        57.4%
        50%
        34.6
        SRR7819859_1
        72.9%
        49%
        36.5
        SRR7819859_2
        69.9%
        50%
        36.5
        SRR7819860_1
        69.5%
        50%
        37.1
        SRR7819860_2
        68.8%
        50%
        37.1
        SRR7819861_1
        70.3%
        50%
        30.7
        SRR7819861_2
        70.4%
        50%
        30.7
        SRR7819862_1
        76.6%
        48%
        56.3
        SRR7819862_2
        73.2%
        49%
        56.3
        SRR7819863_1
        71.4%
        51%
        41.5
        SRR7819863_2
        70.1%
        51%
        41.5
        SRR7819864_1
        73.3%
        51%
        29.9
        SRR7819864_2
        71.9%
        51%
        29.9
        SRR7819865_1
        63.9%
        50%
        45.7
        SRR7819865_2
        67.5%
        50%
        45.7
        SRR7819866_1
        69.4%
        50%
        29.6
        SRR7819866_2
        70.5%
        51%
        29.6
        SRR7819867_1
        69.5%
        50%
        35.9
        SRR7819867_2
        68.1%
        50%
        35.9
        SRR7819868_1
        73.5%
        51%
        30.8
        SRR7819868_2
        69.7%
        52%
        30.8
        SRR7819869_1
        74.0%
        48%
        39.4
        SRR7819869_2
        70.0%
        49%
        39.4
        SRR7819870_1
        66.9%
        50%
        33.2
        SRR7819870_2
        67.4%
        50%
        33.2
        SRR7819871_1
        68.3%
        50%
        38.3
        SRR7819871_2
        66.9%
        50%
        38.3
        SRR7819872_1
        69.9%
        50%
        32.9
        SRR7819872_2
        67.5%
        50%
        32.9
        SRR7819873_1
        72.4%
        48%
        39.2
        SRR7819873_2
        69.8%
        49%
        39.2
        SRR7819874_1
        70.2%
        49%
        50.3
        SRR7819874_2
        67.9%
        50%
        50.3
        SRR7819875_1
        70.2%
        49%
        31.7
        SRR7819875_2
        64.9%
        50%
        31.7
        SRR7819876_1
        62.6%
        50%
        28.5
        SRR7819876_2
        63.2%
        51%
        28.5
        SRR7819877_1
        61.9%
        49%
        33.9
        SRR7819877_2
        57.5%
        49%
        33.9
        SRR7819878_1
        77.0%
        50%
        35.8
        SRR7819878_2
        73.5%
        50%
        35.8
        SRR7819879_1
        72.7%
        49%
        37.8
        SRR7819879_2
        68.2%
        49%
        37.8
        SRR7819880_1
        72.5%
        51%
        34.9
        SRR7819880_2
        70.5%
        51%
        34.9
        SRR7819881_1
        73.9%
        52%
        36.0
        SRR7819881_2
        70.7%
        52%
        36.0
        SRR7819882_1
        74.8%
        50%
        31.7
        SRR7819882_2
        73.2%
        50%
        31.7
        SRR7819883_1
        73.4%
        50%
        37.2
        SRR7819883_2
        68.5%
        50%
        37.2
        SRR7819884_1
        69.6%
        50%
        38.6
        SRR7819884_2
        68.4%
        50%
        38.6
        SRR7819885_1
        66.3%
        50%
        40.2
        SRR7819885_2
        63.5%
        50%
        40.2
        SRR7819886_1
        74.2%
        49%
        47.6
        SRR7819886_2
        71.2%
        50%
        47.6
        SRR7819887_1
        67.1%
        51%
        40.5
        SRR7819887_2
        68.8%
        52%
        40.5
        SRR7819888_1
        66.6%
        50%
        32.4
        SRR7819888_2
        65.5%
        50%
        32.4
        SRR7819889_1
        69.3%
        50%
        52.2
        SRR7819889_2
        67.3%
        50%
        52.2
        SRR7819890_1
        74.7%
        52%
        38.2
        SRR7819890_2
        74.5%
        52%
        38.2
        SRR7819891_1
        73.2%
        51%
        30.2
        SRR7819891_2
        72.0%
        51%
        30.2
        SRR7819892_1
        71.4%
        50%
        33.3
        SRR7819892_2
        71.5%
        51%
        33.3
        SRR7819893_1
        70.7%
        51%
        45.7
        SRR7819893_2
        69.6%
        51%
        45.7
        SRR7819894_1
        71.4%
        48%
        39.6
        SRR7819894_2
        67.9%
        48%
        39.6
        SRR7819895_1
        76.2%
        48%
        41.1
        SRR7819895_2
        70.6%
        49%
        41.1
        SRR7819896_1
        60.1%
        50%
        32.1
        SRR7819896_2
        61.7%
        50%
        32.1
        SRR7819897_1
        67.7%
        50%
        39.7
        SRR7819897_2
        66.0%
        51%
        39.7
        SRR7819898_1
        71.3%
        51%
        50.0
        SRR7819898_2
        70.0%
        51%
        50.0
        SRR7819899_1
        62.5%
        49%
        28.4
        SRR7819899_2
        61.9%
        49%
        28.4
        SRR7819900_1
        69.1%
        51%
        40.1
        SRR7819900_2
        67.8%
        51%
        40.1
        SRR7819901_1
        78.4%
        50%
        57.5
        SRR7819901_2
        78.7%
        50%
        57.5
        SRR7819902_1
        68.0%
        49%
        47.6
        SRR7819902_2
        64.3%
        50%
        47.6
        SRR7819903_1
        71.1%
        49%
        33.0
        SRR7819903_2
        69.2%
        50%
        33.0
        SRR7819904_1
        67.5%
        50%
        45.8
        SRR7819904_2
        68.4%
        51%
        45.8
        SRR7819905_1
        63.3%
        50%
        36.8
        SRR7819905_2
        61.3%
        50%
        36.8
        SRR7819906_1
        62.9%
        51%
        39.3
        SRR7819906_2
        64.3%
        51%
        39.3
        SRR7819907_1
        66.7%
        51%
        31.4
        SRR7819907_2
        66.3%
        51%
        31.4
        SRR7819908_1
        69.9%
        50%
        34.4
        SRR7819908_2
        69.0%
        50%
        34.4
        SRR7819909_1
        69.2%
        50%
        47.6
        SRR7819909_2
        69.7%
        50%
        47.6
        SRR7819910_1
        86.0%
        50%
        76.2
        SRR7819910_2
        86.1%
        51%
        76.2
        SRR7819911_1
        70.0%
        52%
        39.1
        SRR7819911_2
        71.2%
        53%
        39.1
        SRR7819912_1
        64.3%
        50%
        27.0
        SRR7819912_2
        65.0%
        51%
        27.0
        SRR7819913_1
        69.6%
        48%
        55.1
        SRR7819913_2
        68.4%
        48%
        55.1
        SRR7819914_1
        69.3%
        49%
        53.4
        SRR7819914_2
        68.5%
        49%
        53.4
        SRR7819915_1
        61.6%
        51%
        32.6
        SRR7819915_2
        62.8%
        52%
        32.6
        SRR7819916_1
        70.6%
        49%
        34.3
        SRR7819916_2
        70.8%
        49%
        34.3
        SRR7819917_1
        74.4%
        51%
        33.7
        SRR7819917_2
        74.8%
        52%
        33.7
        SRR7819918_1
        68.8%
        49%
        37.0
        SRR7819918_2
        68.8%
        49%
        37.0
        SRR7819919_1
        66.4%
        48%
        29.7
        SRR7819919_2
        62.0%
        49%
        29.7
        SRR7819920_1
        67.0%
        51%
        34.5
        SRR7819920_2
        65.5%
        51%
        34.5
        SRR7819921_1
        74.1%
        54%
        33.3
        SRR7819921_2
        75.0%
        54%
        33.3
        SRR7819922_1
        69.2%
        48%
        46.1
        SRR7819922_2
        62.8%
        49%
        46.1
        SRR7819923_1
        70.0%
        51%
        40.6
        SRR7819923_2
        70.3%
        51%
        40.6
        SRR7819924_1
        62.8%
        50%
        31.1
        SRR7819924_2
        61.2%
        50%
        31.1
        SRR7819925_1
        67.1%
        50%
        34.2
        SRR7819925_2
        67.3%
        51%
        34.2
        SRR7819926_1
        67.9%
        49%
        37.8
        SRR7819926_2
        64.7%
        50%
        37.8
        SRR7819927_1
        83.6%
        52%
        42.3
        SRR7819927_2
        83.7%
        53%
        42.3
        SRR7819928_1
        72.7%
        51%
        42.6
        SRR7819928_2
        74.6%
        51%
        42.6
        SRR7819929_1
        64.8%
        49%
        32.0
        SRR7819929_2
        62.1%
        50%
        32.0
        SRR7819930_1
        64.3%
        49%
        28.2
        SRR7819930_2
        62.4%
        50%
        28.2
        SRR7819931_1
        74.8%
        50%
        45.7
        SRR7819931_2
        76.3%
        50%
        45.7
        SRR7819932_1
        65.6%
        51%
        34.9
        SRR7819932_2
        63.7%
        51%
        34.9
        SRR7819933_1
        66.2%
        50%
        38.7
        SRR7819933_2
        66.0%
        50%
        38.7
        SRR7819934_1
        67.8%
        50%
        66.2
        SRR7819934_2
        64.4%
        50%
        66.2
        SRR7819935_1
        76.4%
        51%
        36.9
        SRR7819935_2
        76.5%
        51%
        36.9
        SRR7819936_1
        67.0%
        50%
        35.1
        SRR7819936_2
        65.3%
        51%
        35.1
        SRR7819937_1
        68.3%
        50%
        40.7
        SRR7819937_2
        66.1%
        51%
        40.7
        SRR7819938_1
        67.1%
        51%
        39.8
        SRR7819938_2
        68.1%
        51%
        39.8
        SRR7819939_1
        67.8%
        51%
        41.9
        SRR7819939_2
        64.5%
        51%
        41.9
        SRR7819940_1
        60.4%
        51%
        38.3
        SRR7819940_2
        59.5%
        51%
        38.3
        SRR7819941_1
        72.4%
        50%
        38.7
        SRR7819941_2
        71.9%
        51%
        38.7
        SRR7819942_1
        71.9%
        52%
        35.8
        SRR7819942_2
        72.0%
        52%
        35.8
        SRR7819943_1
        81.6%
        52%
        76.5
        SRR7819943_2
        81.2%
        52%
        76.5
        SRR7819944_1
        96.6%
        49%
        64.0
        SRR7819944_2
        94.4%
        49%
        64.0
        SRR7819945_1
        66.2%
        52%
        29.7
        SRR7819945_2
        65.8%
        52%
        29.7
        SRR7819946_1
        64.3%
        50%
        31.8
        SRR7819946_2
        62.5%
        50%
        31.8
        SRR7819947_1
        73.2%
        50%
        29.4
        SRR7819947_2
        72.1%
        51%
        29.4
        SRR7819948_1
        59.6%
        49%
        31.4
        SRR7819948_2
        56.2%
        49%
        31.4

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        loading..

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        loading..

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        loading..

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        loading..

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        loading..

        Sequence Length Distribution

        All samples have sequences of a single length (101bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        loading..

        Overrepresented sequences

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        loading..

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        loading..

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        loading..

        Run Time

        Run Time This analysis is about the MultiQC run itself, profiling the time spent on different parts of the MultiQC execution. It is designed to help developers optimise how they run MultiQC, to get the most efficient and fastest configuration possible. For more information, see the MultiQC documentation. .

        Files searched

        Number of files searched by MultiQC, categorised by what happened to them. Total file searches: 19225.

        Note that only files are considered in this plot - skipped directories are not shown.

        Some search patterns do not discard files after they match (shared: true), so it is possible that some files may be double-counted in this plot.

        • Skipped: No match - File was searched, but didn't match any search patterns
        • Skipped: Ignore pattern - File matched a MultiQC ignore pattern (see -x / --ignore / config.fn_ignore_paths)
        • Skipped: Filesize limit - File was skipped because it was too large (see config.log_filesize_limit)
        • Skipped: Symlinks - File was a symlink and skipped (see config.ignore_symlinks)
        • Skipped: Not a file - File could not be read (eg. was a unix pipe or something)
        loading..

        Search patterns

        Time spent running each search pattern to find files for MultiQC modules. Total file search time: 26.61 seconds.

        NOTE: Usually, MultiQC run time is fairly insignificant - in the order of seconds. Unless you are running MultiQC on many thousands of analysis files, optimising this process will have limited practical benefit.

        MultiQC works by recursively looking through all files found in the analysis directories. After skipping any that are too big / binary file types etc, it uses the search patterns defined in multiqc/utils/search_patterns.yaml. These work by matching either file names or file contents. Generally speaking, matching filenames is super fast and matching file contents is slower.

        Please see the MultiQC Documentation for information on how to optimise MultiQC to speed this process up. The plot below shows which search keys are running and how long each has taken to run in total. This should help to guide you to where optimisation is most worthwhile.

        loading..